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Registros recuperados: 29 | |
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Camassola,Marli; Rosa,Letícia O. da; Calloni,Raquel; Gaio,Tamara A.; Dillon,Aldo J.P.. |
Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm-1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pleurotus; Laccase; Manganese peroxidase; Mushroom production; Biotechnological applications. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100031 |
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Cavallazzi,José Renato P.; Kasuya,Catarina M.; Soares,Marcos A.. |
Laccases are enzymes involved in lignin degradation and are produced by various organisms. Due to their low substrate specificity their potential to be used in biotechnological applications has received attention. The addition of laccase inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. The aim of this study was to investigate the effect of some compounds as laccase inducers in cultures of Lentinula edodes (shiitake). First, it was selected a culture medium suitable for laccase production by shiitake using two levels of N (2.6 and 26 mM) and seven levels of Cu (0, 50, 100, 150, 200, 250 and 300 µM). The medium with 2.6 mM N and 250 µM Cu was found to provide the highest laccase... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Shiitake; Laccase inducers. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400015 |
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Diez J,M. Cristina; Gallardo A,Felipe; Saavedra,Gisselle; Cea L,Mara; Gianfreda,Liliana; Alvear Z,Marisol. |
The activities of laccase, Mn-peroxidase, phosphatase and dehydrogenase were studied in a Chilean Andisol (Temuco Serie) contaminated with pentachlorophenol (PCP). Experiments were conducted on the top of 0-20 cm soil, adding different concentrations of PCP (0.41, 4.1 and 41 mg kg-1). Enzymatic activities and residual concentrations of PCP were evaluated in soil incubated at 25 ± 1 ºC for 112 days. Enzyme activities of PCP-treated soil varied according to the PCP concentration added. For instance, an inhibitory effect on Mn-peroxidase activity was not observed during incubation at the three levels of PCP, whereas acid phosphatase activity was only affected by the 0.41 mg kg-1 PCP treatment. By contrast, laccase and dehydrogenase activities were negatively... |
Tipo: Journal article |
Palavras-chave: Pentachlorophenol; Andisol; Laccase; Mn-peroxidase; Phosphatase; Dehydrogenase. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912006000300004 |
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Feng,Bao Zhen; Li,Peiqian. |
Laccases are blue copper oxidases (E.C. 1.10.3.2) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O. A novel laccase gene pclac2 and its corresponding full-length cDNA were cloned and characterized from Phytophthora capsici for the first time. The 1683 bp full-length cDNA of pclac2 encoded a mature laccase protein containing 560 amino acids preceded by a signal peptide of 23 amino acids. The deduced protein sequence of PCLAC2 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. In order to achieve a high level secretion and full activity expression of PCLAC2, expression... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phytophthora capsici; Laccase; Expression; Purification; Activity. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100050 |
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Fonseca,María Isabel; Tejerina,Marcos Raúl; Sawostjanik-Afanasiuk,Silvana Soledad; Giorgio,Ernesto Martin; Barchuk,Mónica Lucrecia; Zapata,Pedro Darío; Villalba,Laura Lidia. |
Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White rot fungi; Trametes; Laccase; Manganese peroxidase; Cu2+ response. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
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Gomes,Eleni; Aguiar,Ana Paula; Carvalho,Caio César; Bonfá,Maricy Raquel B.; Silva,Roberto da; Boscolo,Mauricio. |
Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Manganese peroxidase; Lignin peroxidase; Basidiomycetes; Decolorization; Synthetic dye. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100005 |
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Jia,Honghua; Gao,Zhen; Ma,Yingying; Zhong,Chao; Xie,Yingchun; Zhou,Hua; Wei,Ping. |
Background: Enzymatic decolourization has been recently proposed as a promising and eco-friendly method for treatment of synthetic dye-contaminated wastewaters. However, the processes require large quantities of enzymes, attracting significant attention in developing efficient methods for mass production of multifunctional enzymes. Several methods such as response surface methodology (RSM) and orthogonal experiment have been applied to optimize the parameters in bioprocesses for enzyme production. Results: In the present study, a laccase-like enzyme, phenoxazinone synthase (PHS) originated from Streptomyces antibioticus was recombinantly expressed in Escherichia coli BL21 (DE3). The production of PHS in E. coli BL21 was optimized by response surface... |
Tipo: Journal article |
Palavras-chave: Congo red; Decolourization; Laccase; Phenoxazinone synthase; Response surface methodology. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500002 |
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Levin,L.; Ramos,A .M.; Parisi,M .; Gally,M.. |
Colletotrichum truncatum is the most common pathogen fungus associated with soybean anthracnose. Although the lignin-degrading enzyme laccase has been implicated in pathogenicity of a wide range of plant pathogenic fungi, its biological role in the Colletotrichum -soybean disease system is unknown. The extent of the infection in our country led us to examine laccase production in Argentinean Colletotrichum strains isolated from diseased soybean plants from different geographic locations. Ten strains (eight of them identified as C. truncatum) , were screened for in vitro laccase production. Only six of the isolates, all of them C. truncatum , produced laccase activity when cultured on a defined medium based on pectin and asparagine as carbon and nitrogen... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthracnose; Colletotrichum; Laccase; SDS-PAGE; Soybean. |
Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100010 |
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Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco. |
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides... |
Tipo: Text |
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf |
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Luz,José Maria Rodrigues da; Nunes,Mateus Dias; Paes,Sirlaine Albino; Torres,Denise Pereira; Silva,Marliane de Cássia Soares da; Kasuya,Maria Catarina Megumi. |
The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Cellulase; Mushrooms; Lignocellulolytic residues. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035 |
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Maciel,Márcia Jaqueline Mendonça; Silva,Ademir Castro e; Ribeiro,Helena Camarão Telles. |
Ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. The demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. Their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. This paper reviews the applications of ligninolytic... |
Tipo: Journal article |
Palavras-chave: Laccase; Lignin peroxidase; Manganese peroxidase; White-rot fungi. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600014 |
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Mikiashvili,Nona A; Isikhuemhen,Omoanghe S; Ohimain,Elijah I. |
Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grifola frondosa; Exopolysaccharide; Laccase; Lignin degradation; Peroxidase. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300031 |
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Registros recuperados: 29 | |
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